Intracytoplasmic Sperm Injection (ICSI) in B6D2F1 and CB6F1 Strains Mice Using Cauda Epididymal Spermatozoa

نویسندگان

چکیده

Objective: Reproductive biotechnology studies focus on the longterm storage of embryos (cryopreservation), embryo cultures, genome editing and transfer. Micromanipulation techniques in reproduction biotechnologies have an important role, especially investigating assisted reproductive technology laboratory animals. The aim present study was to investigate effect epididymal spermatozoa injected oocyte by intracytoplasmic sperm injection (ICSI) different mice strains. In this study, we evaluated vitro development post-ICSI derived using cauda sperm. Material Method: Female (8-10 weeks) were superovulated pregnant mare serum gonadotropin/human chorionic gonadotropin (PMSG/hCG) ~14h post hCG, sacrificed, oocytes collected. Spermatozoa from a 12-week-old used same strain for ICSI developmental potential evaluated. Finally, cultured 120 hours at 5% CO2 with 37°C. Results: results showed that two-cell B6D2F1 (79.31%) significantly higher than CB6F1 (56.26%) (p<0.05). While blastocyst rate comparable between both (68.75%) (69.57%) (p>0.05). Conclusion: is suitable ap plication success strains found be

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ژورنال

عنوان ژورنال: Experimed

سال: 2021

ISSN: ['2667-5846']

DOI: https://doi.org/10.26650/experimed.2021.984139